There are always new updates in regards to science as researchers never stop working to figure out better ways to do things. In the field of research, regular updating is one of the most constant things, and there are a lot of different equipment, particles, and substances used in the processes they do.
Dulbecco’s PBS and PBS are one of those substances that are important in the research process. They are water-based solutions whose purpose is to maintain consistent pH in both biological and chemical applications, which are needed for different experiments and tests.
People often get confused between the two since they are both water-based and colorless solutions that help in tissue building. However, there are actually a lot of differences between the two of them that would be helpful in differentiating them.
Dulbecco’s PBS vs. PBS
The main difference between Dulbecco’s PBS and PBS would be that even if they are both used in biological research, they have different compositions from one another. Dulbecco’s PBS might not have calcium or magnesium in its contents, while the standard or regular PBS does not have them at all.
Dulbecco’s PBS is a buffer solution essential in acid chemistry. It is a solution that has a highly stable PH and has a lot of different uses, such as dilution of different substances, rinsing of instruments, and a whole lot more. This is why it is very important to know what it is composed of and what it is used for so that you can be sure to use it properly.
On the other hand, the standard PBS solution mostly contains disodium hydrogen phosphate and sodium chloride, and it plays a very important role in cell biology that helps in preventing the rupture and shriveling of the cell. This means that using this substance would not destroy the cell in any way, such as shrinking or wrinkling it. Overall, it does not mess with any part of the experiment, which is considered very important in any research.
What is Dulbecco’s PBS?
Dulbecco’s PBS is a water-based solution that is used mostly in biological research. It is a colorless solution that is particularly used in experimenting so that the cell would not expand or contract. Another purpose of the solution is to clean the cell and preserve the cell by using it with other substances.
One of the things that makes it different from the standard PBS would be the concentration of the phosphate that is in it. It has a lower phosphate concentration than that of the standard PBS. Like PBS, it is also compatible with the human body. Dulbecco’s PBS, which does not contain calcium nor magnesium, is known as the CMF-PBS or the calcium-magnesium-free PBS.
The base solution of Dulbecco’s PBS includes the following: potassium phosphate, sodium phosphate, sodium chloride, and potassium chloride. In some experiments, it also has calcium and magnesium. It can also include phenol red depending on the person that is using it. Overall, it is a balanced salt solution. Its recommended storage would be at a temperature of two to eight degrees Celsius. When the solution is turbid, which is when the liquid turns into a thicker substance and looks very cloudy, it is then that it should be discarded.
What is PBS?
PBS is quick writing of the word phosphate-buffered saline. It is a substance mostly used for the processing of tissue and for pH maintenance. It is isotonic to humans, which means that it is safe to use since it comes with fewer chances of being toxic or causing any damage to the cell during research or the conduction of experiments.
Though they happen to be safer than the other chemicals in terms of use, it is still recommended not to use them for a long time since by doing that; the cells might die due to over-stressing. Thus, it is necessary to keep time in check when you are using this substance.
The main components of the standard PBS are the following: sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. Besides maintaining the level of pH in a solution, it is also used to clean containers, dry molecules, and dilute specific solutions.
Since it is known to be isotonic in the human body, it means that the concentration of it is safe to use for the body. The standard PBS is of many uses in a lot of different experiments, and that is the very reason why it is important to learn more about it.
Difference Between Dulbecco’s PBS and PBS
- The concentration of the substances found in Dulbecco’s PBS is different from those that are in the standard PBS. Dulbecco’s PBS has a lower phosphate concentration than the standard PBS.
- Dulbecco’s PBS might or might not have magnesium or calcium as an additive to it, while the standard PBS totally does not have both of them.
- The main use of Dulbecco’s PBS is to be used in the cell containing experiments and making sure that it does not expand or shrink. On the other hand, the standard PBS is used to maintain the solution at a constant pH level.
- The main components of PBS include disodium hydrogen phosphate and sodium chloride, while Dulbecco’s PBS has a different composition.
- Dulbecco’s PBS can be used together with phenol red, while regular PBS does not use it at all.
- The standard pH level of Dulbecco’s PBS is 7.2 to 7.6, while standard PBS is at 6.8 to 7.4
Conclusion
Though both substances, Dulbecco’s PBS and PBS can look quite the same in terms of appearance, they highly differ in everything else. They have different maintaining temperatures, pH, and components. This means that the person who will be performing an experiment needs more knowledge and precaution when using either of them.